Sugarcane, somaclonal variations, red rot, scmv, candidate genes, genetic integrity. The occurrence of somaclonal variation can be reduced by. Pdf assessment of genetic variability in somaclonal. In vitro generation of somaclonal variant plants of sugarcane saccharum spp. Somaclonal variation is a tool that can be used by plant breeders.
Feb 01, 20 read in vitro generation of somaclonal variant plants of sugarcane for tolerance to fusarium sacchari, plant cell reports on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Pdf somaclonal variation as a tool for crop improvement. Ja605 genotypes expressing a tcrylab gene introduced by cell electroporation were evaluated under severe borer diatraea saccharalis fab. Feb, 2016 however, plant tissue culture may generate genetic variability, i. It is well known that increasing numbers of subculture increases the likelihood of somaclonal variation, so the number of subcultures in micropropagation protocols. Tissue culture is an efficient method of clonal propagation. Sugarcane growing countries of the world are lying between the latitude 36. The use of only one type of molecular marker, such as rapds, to assess the genetic stability of an in vitro production system may be inadequate, and an approach that focuses on morphological traits appears to be a valuable. Studying the somaclonal variation within transgenic sugarcane populations and cultured cells. Read this article to learn about the basis, isolation and limitations of somaclonal variations and also factors affecting the production of somaclonal variants. Somaclonal variations was a good source of genetic improvement of sugarcane for disease resistance with no snp changes in candidate genes. In vitro regeneration, detection of somaclonal variation and screening for mosaic virus in sugarcane saccharum spp. Pdf to text batch convert multiple files software please purchase personal license.
Tissue cultureinduced somaclonal variation of decreased. Somaclonal variation, crop improvement, plant tissue culture. Somaclonal variation an overview sciencedirect topics. Crop science abstract evaluation of in vitro propagated. Study of somaclonal variation in two rice cultivars regenerated from embryo culture, using ssr markers hamed salehian 1, nadali babaeian 1, nadali bagheri 1, behnam sedaghati 2, farzad banaeiasl 3, sara kabirnataj 1 1department of plant breeding and biotechnology, sari agricultural sciences and natural university, iran. Iurure applications in the next five years, somaclonal variation should become widely used for crop improvement. Eight genotypes of saccharum officinarum were crossed with saccharum spontaneum and 14 genotypes of s. Somaclonal variation a novel source of variability from. Application of somaclonal variation in crop improvement programme was established in sugarcane. Reducing somaclonal variation different steps can be used to reduce somaclonal variation. The field grown somaclones were characterized for yield and quality. Similarly, genetically transformed cells are carefully screened to avoid unwanted and unintended somaclonal variations so that the commercially released clones have only beneficial effects. Saurabh bhatia, kiran sharma, in modern applications of plant biotechnology in pharmaceutical sciences, 2015.
Keywords callus epigenetic genetic regeneration somaclonal variation. Sep 23, 20 phenotypic and genotypic variations, collectively called somaclonal variations, are induced during tissue culture. The possible mechanisms causing somaclonal variation. Regeneration and evaluation of somaclones of sugarcane variety. Genetic variations in plants that have been produced by plant tissue culture and can be detected as genetic or phenotypic traits. Pdf cell and tissue culture approaches serve as an important and an easily accessible source for the creation and utilization of variability in. Read somaclonal variation as a source of resistance to eyespot disease of sugarcane, plant breeding on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Assessment of somaclonal variation during sugarcane. According to the increasing demand of sugarcane somaclonal variation play vital role to overcome problems for sugarcane breeding. But it is disadvantageous when the objective is micropropagation of considered genotypes. Although the direct formation of plant structures from meristem cultures, without any. The usefulness of variation was first demonstrated through the recovery of disease resistant plants in potato resistance against late blight and early blight and sugarcane resistance against eyespot disease, fiji disease and downy. This document summerizes results obtained since the 1970th, on plants regenerated through in vitro tissue culture, and for which a large variability was expressed. Somaclonal variation is the variation seen in plants that have been produced by plant tissue culture.
Somaclonal variation it is the term used to describe the variation seen in plants that have been produced by plant tissue culture. Somaclonal variation free download as powerpoint presentation. Somaclonal variation for sugarcane improvement springerlink. The tech niques are more simple than those of recombinant dna, and they result in a rich source of genetic vari ability. This article throws light upon the five applications of somaclonal variations. Article induction and evaluation of somaclonal variation in sugarcane. Considering the sources of somaclonal variation, it is unpractical the. We studied the phenotypic variation in pollen viability of regenerants of torenia after subculturing for one to nine generations. Ii growth and production of sugarcane willy verheye encyclopedia of life support systems eolss sugar cane is a perennial grass that thrives well in tropical and frostfree warm temperate areas. Somaclonal variation in tissue culture is a complex problem that needs several approaches to be appreciated correctly. Advantages somaclonal variations are stable and occur at high frequencies. Somaclonal variation and in vitro mutation induction have been used in sugarcane, banana and potato, with the objective of improving specific characters, mainly the resistance to diseases and other defects that limit the use of some important commercial varieties.
Somaclonal variation induced through tissue culture, first observed in sugarcane saccharum, has been reported in many other plant species. A population of transgenic sugarcane saccharum hybrid cv. Box 18, chiuju, pingtung taiwan abstract an improvement program for disease resistance and horticultural traits in cavendish banana cultivars was established using somaclonal variants as a source of variation. Avoiding the somaclonal variation in the production of transgenic sugarcane plants. Genetic variation found to occur between somaclones in plant tissue cultures was called somaclonal variation. Assessing somaclonal variation in rye, linacero et al. These somaclonal variations are mainly caused by newly generated mutations arising from tissue culture process sato et al. Journal of food health and bioenvironmental science. Pdf in vitro regeneration, detection of somaclonal. Heritable, genetic changes alter the dna stable, but nonheritable changes alter gene expression, aka epigenetic since. Yld of sugarcane is caused by sugarcane yellow leaf virus. A case study with olive 125 whereas in vitro the loss of cellular control, which gives rise to disorganised growth, is a characteristic of somaclonal variation 6,19.
Somaclonal variants of several crops have been successfully obtained by this approach e. It is argued that this variation already is proving to be of. Somaclonal variations may reduce two years the time required for the release of new variety compared to mutation breeding. Desirable agronomic traits and released cultivars have even been derived from this type of induced variation 7. The term somaclonal variation is a phenomenon of broad taxonomic occurrence, reported for species of different ploidy levels, and for outcrossing and inbreeding, vegetatively and seed propagated, and cultivated and non. In india, a somaclonal variant of citronella java, a medicinal plant has been released as bio for commercial cultivation by central institute for medicinal and aromatic plants cimap, lucknow.
Study of somaclonal variation in two rice cultivars. However, it should be remembered that variation and mutations can also occur during in vivo propagation orton, 1984. Applications of somaclonal variations biology discussion. Plant tissue culture or micropropagation technique is the rapid method to multiply newly released cultivar in limited time. Pdf somaclonal variation in sugarcane through tissue culture and. It requires high temperatures, plenty of sunlight, large quantities of. Genomic variation and dna repair associated with soybean. Somaclonal variation for sugarcane improvement pooja manchanda, ajinder kaur, and satbir singh gosal abstract cell and tissue culture approaches serve as an important and an easily. In this article, we will discuss about the isolation and selection techniques of somaclonal variation. Somaclonal variations occurred among the plants regenerated through somatic embryogenesis. Somaclonal variation for disease resistance in wheat and production of dihaploids through wheat x maize hybrids.
Rapd markers were also employed to identify sugarcane somaclonal variants resistant to sugarcane mosaic virus oropeza et al. Advantages of somaclonal variations help in crop improvement creation of additional genetic varitions increased and improved production ofsecondary metabolites selection of plants resistant to varioustoxins, herbicides, high salt concentrationand mineral toxicity suitable for breeding of tree species. The molecular underpinnings of somaclonal variation can include dna sequence changes, chromosome. View and download powerpoint presentations on somaclonal variation ppt. Due to limited genetic system andor narrow genetic base, somaclonal variation is more rewarding in sugarcane. Somaclonal variations and their applications in horticultural.
Saieed introduction substantial genetic and phenotypic variation occurs within plant cell, protoplast, anther, or tissue cultures. Induced mutations and somaclonal variation in sugarcane. Somaclonal variation in sugarcane various factors responsible for somaclonal variation which include karyotype changes, cryptic changes associated with chromosome rearrangement,transposable elements, somatic gene rearrangements,gene amplification and depletion, somatic crossing over and sisterchromatoid exchanges. Find powerpoint presentations and slides using the power of, find free presentations research about somaclonal variation ppt. Somaclones of nia1198 were developed through callus culture and subjected to field trials under randomized complete block design with three replications over two consecutive years 2014 and 201415. Somaclonal variations have largely contributed towards the development of disease resistance in many crops e. In this study, using intersimple sequence repeat issr molecular markers, the somaclonal variation during micropropagation of sugarcane using temporary immersion bioreactors tibs was evaluated.
Pdf eight genotypes of saccharum officinarum were crossed with saccharum spontaneum and 14 genotypes of s. Selected crops somaclonal variants, with increased disease resistance developed, without in vitro selection and within vitro selection are respectively given in tables 46. During plant propagation process under laboratory conditions, the genomic shock is induced which can result in the activation of various transposons, retrotransposons, chromosomal changes, methylation and demethylation of dna leading. Crop science abstract evaluation of in vitro propagated sugarcane hybrids for somaclonal variation 1 view my binders this article in cs. This site is like a library, use search box in the widget to get ebook that you want. Somaclonal variation in vitro culture techniques plant. Somaclonal variation is not restricted to, but is particularly common in plants regenerated from callus. Co 94012 is a somaclonal variant of coc 671, with better sucrose content and moderately resistant to red rot and smut diseases. An understanding of the molecular nature and mechanisms of somaclonal variation is important to evaluate the background journal of food health and bioenvironmental science mayaugust 2018, 112. Assessment of genetic variability in somaclonal population of sugarcane. Somaclonal variation in arracacha is a result of plant regeneration via indirect morphogenesis and can be detected by issr markers. Angra 2 1 instituto agronomico do paranaiapar, caixa postal 481, 8600970 londrina, pr, brasil. Somaclonal variation in crop improvement ii download.
Phenotypic and dna variation among putative plant clones is termed somaclonal variation. In the present investigation, optimal media compositions for enhancing the callus induction and subsequent plant regeneration were worked out in sugarcane saccharum spp. Cluster analysis, principal component analysis, somaclonal variations, sugarcane, tissue culture. A study was conducted from 2006 to 2008 to assess the variability arising from callus regeneration and its vegetative transmission in a. Control of somaclonal variation now the question arises, how to control somaclonal variation or in other words how to make this approach more reliable so that plant breeder can make regular use of it in crop improvement. The aim of this study to evaluate genetic variation developed through soma clones and for this purpose three sugarcane. In this article, we will discuss about the possible mechanisms causing somaclonal variations. Somaclonal variation and crop improvement larkin and scowcroft 1981 proposed the term somaclone to describe the plants originating from any type of tissue culture. In vitro generation of somaclonal variant plants of. Somaclonal variation can be manifested as either somatically or meiotically stable events. We found that pollen viability of regenerants continuously decreased with increasing subculture time. Variation in plant phenotype is determined by genetic and epigenetic factors.
Advantages of somaclonal variations help in crop improvement creation of additional genetic varitions increased and improved production of secondary metabolites selection of plants resistant to various toxins, herbicides, high salt concentration and mineral toxicity, and disease resistance. Sugarcane variety co 94012 is a new variety released in india through the use of somaclonal variation. Co 94012 a new sugarcane variety through somaclonal variation. Mutation breeding as a methodology for crop improvement is based on the possibility of altering genes by exposing vegetative propagules, gametes or seeds to. The hawaian sugar planters association experimental station recorded genetic variation among sugarcane plants regenerated from tissue culture. When genetic variation is observed in plants which have been regenerated in vitro often the result of genetic instability, then the term somaclonal variation is used scowcroft, 1985. Sugarcane improvement through biotechnology authorstream.
Somaclonal variation for disease resistance in wheat and. Nevertheless, the genetical analyses of the peculiar regenerants led to uncommon results that make think to the. Because there is a chance of result of unwanted offtypes that reduces the commercial value. Somaclonal variation is the introduction of genetic variation in in vitro cultured cells and plants derived from such cells are called. Extensive examples are discussed of such variation in culture subclones and in regenerated plants somaclones. Application of tissue culture variability to crop improvement. In india, sugarcane breeding institute, coimbatore has released varieties produced through the process of somaclonal variation. Selected crops somaclonal variants, with increased disease resistance developed. Assessment of somaclonal variation in sugarcane semantic scholar.
Transformation of foreign gene in sugarcane variety isd 28 using agrobacterummediated method. Dna methylation in sugarcane somaclonal variants assessed. Somaclonal variations considered a novel method of genetic variation and having much interest as a basic genetic process, as it contradicts the theory or clonal uniformity. A selected list of disease resistant crop plants obtained from somaclonal variations without in vitro selection along with the pathogenic organisms is given in table 46. Sources of variations detected in plant tissue culture. Somaclonal variation includes aneuploids, sterile plants and morphological variants, sometimes involving traits of economic importance in case of crop plants. Variations were recorded based on cytogenetic, morphological and enzyme profiles. The occurrence of subtle somaclonal variation is a drawback for both in vitro cloning as well as germplasm preservation. The plants derived from such cells are referred to somaclones.
The occurrence of somaclonal variation is both a boon and bane. This map depicts the distribution of sugarcane in the world. Somaclonal variation caused by the process of tissue culture is also called tissue culture induced variation to more speci. In addition, somaclonal variation has been free of the regulatory hurdles that plague products of recombinant dna. Genetic variations in plants thathave been produced by plant tissueculture and can be detected asgenetic or phenotypic traits. Somaclonal variation and in vitro mutation induction. Epigenetic aspects of somaclonal variation in plants. The genetic variations found in the in vitro cultured cells are collectively referred to as somaclonal variations. Most of the early works to elucidate the genetic basis of somaclonal variation were done on sugarcane and potato. Assessment of somaclonal variation in indirect morphogenesis. Somaclonal variants resistant to sugarcane mosaic virus scmv were obtained from susceptible sugarcane cv pr62258 through somatic embryogenesis by increasing the number of subcultures of the embryogenic callus tissue in ms medium with 3 mgl 2,4dichlorophenoxyacetic acid. Two somaclones, namely at626 and bt627, were selected.
These clones have different discard percentages and were categorized according to the in vitro visual frequency of somaclonal variation symptoms abnormal in vitro plantlets in two groups. It can be concluded from the study that somaclonal variations can create highly diverse populations of sugarcane for evaluation in cane breeding programs. Sugarcane is commercially important and major cash crop of pakistan. These 39 hybrids were raised in the field and used as donor clones for in vitro culture studies. Click download or read online button to get somaclonal variation in crop improvement ii book now.
In sugarcane, variations can be created through tissue culture. Chromosomal rearrangements are an important source of this variation. Naturally occurring variation is a wellestablished source of novel phenotypes in many vegetatively propagated fruits and vegetables, where they are commonly known as sports. Transfers were made at 30day intervals for 1, 2 or 3 subcultures. In vitro generation of somaclonal variant plants of sugarcane. An assessment of somaclonal variation in micropropagated plants of. Tissue culturederived variation in crop improvement pdf. A number of possible mechanisms for the origin of this phenomenon are considered. Somaclonal variation refers to the genetic and epigenetic changes in plants regenerated from plant tissue culture. Somaclonal variants of sugarcane are available for several traits like drought, salt.
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